Coding

Part:BBa_K4317009:Design

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-11)


KsCel7A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NcoI sites are on the front side of this part, and the XhoI site is on the back side.

The stop codon has been removed.

This part was codon-optimized for bacillus subtilis.


Source

This part originated from Kocuria palustris, and we synthesized this DNA fragment from IDT.

References